Gotaq dna 5x green gotaq 5x colorless gotaq magnesium chloride cat. In light of the suspension of business activities in singapore, promega singapore. Has anyone tried to get rough estimation of concentration of a pcr. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel loading dye, purple 6x, no sds. The 1kb dna ladder marker consists of nine double 1 kb dna ladder cat. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. Or only rna molecular weight marker should be used to see rna integrity. Quality and stability of dna ladders shipped at ambient temperature. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination.
The 10 bands of the ladder contain fragments following sizes. Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. These ladders consist of equimolar mixtures of dna fragments for determining the mass of unknown dna samples on gels in the low and high molecular weight ranges. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. How can i prepare working solution with 1 kb dna ladder. The benchtop 1kb dna ladder has bluntended fragments with sizes. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Instructions for use of products j1250, j1255, j3011, j3015, j4011, j4015.
As an essential business serving the needs of the education and healthcare communities, we will be continuing to process and ship orders as well as provide ongoing customer service support. The dna ladder consists of proprietary plasmids and dna molecules which are digested to completion with appropriate restriction enzymes to yield bands suitable for use as molecular weight. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. The ladder consists of nine bands in even increments 0. The 1k and 3k bands have increased intensity to serve as. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The quickload 1 kb extend dna ladder is a premixed, readytoload molecular weight dna marker containing bromophenol blue dye as a tracking dye.
View more information about our dna stains technology. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The dna ladder consists of proprietary plasmids and dna molecules which are digested to completion with appropriate restriction enzymes to yield bands suitable for use as molecular weight standards for agarose gel electrophoresis. These ladders are sometimes made from restriction enzyme digestion of plasmid dna with a known sequence. Is there any free software that i can use to download and view the files. The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free. Invitrogen trackit 1 kb plus dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. The ladder is dephosphorylated and ready for 5 endlabeling with radioisotopes using t4 polynucleotide kinase, allowing visualization by autoradiography. Dna ladders contain dna fragments of varying sizes. Download a pdf containing pricing for our full product list. Certificate of analysis gotaq flexi dna polymerase.
The extension time should be at least 1min kb target length. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Promega dna ladder molecular weight markers, 100bp dna. Is it ok to use dna molecular weight marker in rna electrophoresis. Ultraranger 1kb dna ladder 300bp 24000bp size determination of digested dna with high molecular weight more 100bp and 1 kb ladders are offered from our partner biovision. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. Our dna laddersmarkers are readytouse high quality yet. Promega 1kb dna ladder molecular weight marker 1kb dna ladder. The benchtop 100bp dna ladder has 11 fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. Promega manufacturing and delivery systems continue to be fully. Initial denaturation of longer than 2 minutes at 95c is unnecessary and may reduce yield. The 1kb dna ladder marker consists of nine double 1 kb dna.
Is it ok to use dna molecular weight marker in rna. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. Promega 1kb dna step ladder molecular weight marker for. The approximate mass of dna in each of the bands is provided assuming a 0. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Hyperladder 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more pcr products, especially designed for easy size determination of linear doublestranded dna fragments on 1% to 2% tae or tbe agarose gels. If samples need to be diluted, use te or other buffer of minimal ionic strength. Dna mass ladders are specifically created for quantitative estimation of dna mass in gels. Often, dna molecular weight markers and ladders come in two formats. Promega dna ladder molecular weight markers, 100bp dna ladder. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. How can i prepare working solution with 1 kb dna ladder supplied by promega. Trackit 1 kb plus dna ladder thermo fisher scientific.
The digested dna includes fragments ranging from 0. The benchtop dna markers are supplied in a stabilizing solution of 1x blueorange loading dye, making the markers ready to load. The state of illinois has declared a mandatory shutdown which is currently in place. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Recommended thermal cycling conditions for gotaq dna polymerasemediated pcr amplification. Ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. If you really want to include marker dna fragments with dna amount, there are such. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
Generuler 1 kb plus dna ladder thermo fisher scientific. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Highranger plus 100bp dna ladder 100bp 0bp analyzing dna over a wide range of sizes. The internal lane standard 500 marker is used in each gel lane or capillary injection to increase the precision of analysis and allelic ladders for all 16 loci are provided to simplify interpretation. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. Promega 1kb dna step ladder molecular weight marker for electrophoresis ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. I preferred this product because it has premixed dye. Fill out our technical support form, email us, or call 18006327799. If you load 6 ul of 1 kb ladder promega 600 ng6 ul or 100 ngul ds dna.
Promega 1kb dna ladder molecular weight marker 1kb dna. This marker is ideal for the size determination of digested dna. The 500bp fragment is present at increased intensity for easy identification. The pd1pdl1 blockade bioassay is a bioluminescent cellbased assay that can be used to measure the potency and stability of antibodies and other biologics designed to block the pd1pdl1 interaction. The 1 kb and 3 kb bands contain more dna to provide internal orientation. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and. Additional studies show interpretable results can be obtained with 0. The ladder is composed of fifteen chromatographypurified individual dna. Dna marker bp 1 kb blue is designed for use as molecular weight standard for agarose gel electrophoresis availibility of sample size may be limited. The dna includes fragments ranging from 25010,000 base pairs. Our teams are in regular contact with suppliers and distributors worldwide to manage inventory of raw materials to ensure continued availability. This product is available in quickload purple format enjoy the convenience of a readytoload format. Dna marker bp 1 kb is designed for use as molecular weight standard for agarose gel electrophoresis features.