For competitive elisa assay, serum vitamin d3 test, enzyme immuneassay test, consider the very best in this business. It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit. The assay system utilizes a mouse monoclonal antiprolactin antibody for solid phase microtiter wells immobilization and another mouse monoclonal antiprolactin antibody in the antibodyenzyme horseradish peroxidase conjugate solution. Mouse anti prolactin antibodycoated breakapart well all the reagents within the kit are calibrated for the direct determination of prolactin in human serum. Samples and the prolactinhrp conjugate are added to the wells, where prolactin in. Clinical significance prolactin is a polypeptide hormone synthesized and secreted by the adenohypophysis anterior pituitary gland and the placenta.
The antibodies developed for the test will determine a minimal concentration of human prolactin of 0. The prolactin elisa kit used in this study is an enzyme immunoassay for the detection of canine prolactin in serum developed by demeditec diagnostic gmbh kiel, germany. Rat prolactin elisa kit is used in determination of rat prolactin in cell culture supernatants, plasma, and serum samples. Standard protein item c 2 vials of mouse prolactin. Prolactin has been reported to be a remarkable index of stress response, both acute and chronic, in several species. The drg prolactin elisa kit is a solid phase enzymelinked immunosorbent assay elisa based on the sandwich principle. We measured random samples of human prolactin elisa kit picokine within the same batchlot to ensure the consistency of the kits performances. This research aimed to investigate the possibility of using a commercial elisa enzymelinked immunosorbent assay kit for. Is equine prolactin measurable using a commercially available canine prolactin elisa kit. The eagle biosciences prolactin elisa assay kit for quantitative determination of prolactin concentration in human serum. Abcam s prolactin mouse elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of mouse prolactin in serum, plasma and cell culture supernatants this assay employs an antibody specific for mouse prolactin coated on a 96well plate. The mouse prolactin prl elisa quantitates ms prl in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant ms prl.
The prolactin elisa kit is a colorimetric immunoassay kit with results in 3 hours. The prl human elisa kit is intended for the quantitative determination of prolactin in human serum. Prolactin prl is a 199 aa, 23kd peptide hormone that is secreted primarily by the pituitary gland in both males and females, though its major roles are in pregnancy and lactation. Animals free fulltext determination of prolactin in. A standard curve should be generated for each set of samples assayed. Prolactin elisa assay kit is intended for research use only and not to be used in diagnostic procedures. Elisa inter assay consistency is measured using wells from different plates from the same batch productionlot. Mouse elisa kits canine elisa kits chicken elisa kits assay kits magnetic beads antibodies protein services support promotion events contact home elisa human prolactin elisa kit. Rat prolactin elisa kit for cell culture supernatants. The mouse prolactin elisa kit accurately measures natural mouse prolactin levels quantified versus standard curves obtained and is based on antibodies.
The prolactin quantitative test is based on a solid phase enzymelinked immunosorbent assay elisa. The samples, and antiprolactin hrp conjugate are added to the wells coated with monoclonal antibody to prolactin. Samples and the prolactin hrp conjugate are added to the wells, where prolactin in. Abcam s prolactin in vitro competitive elisa enzymelinked immunosorbent assay kit is designed for the accurate quantitative measurement of prolactin in human serum.
Human prolactinreleasing peptide prrp has been identied as an endogenous ligand for gpr10, and occurs as 31 and 20 amino acid forms. Biovisions prolactin kit is a solid phase direct sandwich elisa kit. Clinical significance prolactin is a polypeptide hormone synthesized and. This assay employs an antibody specific for mouse prolactin coated on a 96well plate. There are currently no images for prolactin elisa kit ka0923. Caymans prolactin human elisa kit is an immunometric i. Browse our prolactin elisa kits elisa kits antibodies. For elisa kit, 1 day storage at 37c can be considered as 2 months at 4c, which means 3 days at 37c equaling 6 months at 4c.
The bosterbio onestep human prolactinprl precoated elisa enzymelinked immunosorbent assay kit is a solid phase immunoassay specially designed to measure human prolactinprl with a 96well strip plate that is precoated with antibody specific for prolactinprl. The human prolactin elisa quantitates human prolactin in human serum, plasma, buffered solution, or cell culture medium. Mouse, rat prolactin elisa kit colorimetric ka0923. The prolactin elisa kit is a solid phase enzymelinked immunosorbent assay elisa based on the sandwich principle. The use of this canine prolactin elisa kit is not validated and thus not. The kit is not calibrated for the determination of prolactin in saliva, plasma or other specimens of human or animal origin. Prolactin in the sample binds to antiprolactin mab on the well and the antiprolactin hrp then binds to prolactin.
Kt52493 intended use this prl elisa kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. Human prolactin elisa kit colorimetric nbp260128 these standard curves are provided for demonstration only. Compare prolactin elisa kits and find the right product on. The microtiter wells are coated with a monoclonal mouse antibody directed towards a unique antigenic site on a prolactin molecule. Rat prolactin elisa kit for cell culture supernatants, plasma. Bovine prl prolactin elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 0. Abcam s prolactin mouse elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of mouse prolactin in serum, plasma and cell culture supernatants. Wash buffer concentrate 20x item b 25 ml of 20x concentrated solution. Purpose the kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of prolactin in bovine serum, plasma and other biological fluids. Background human prolactin lactogenic hormone is secreted from the anterior pituitary gland in both men and women.
For this research, 2 kits were used and the assay was performed according to the manufacturers instructions. Measure up to 38 samples in duplicate per 96 well kit. There is no crossreactivity with hcg, tsh, lh, fsh, or hgh. Every product we sell is backed by novus 100% guarantee. Pdf is equine prolactin measurable using a commercially. Caymans human prolactin elisa kit is an immunometric i. Results obtained using natural human prolactin showed linear curves that were parallel to the standard curves obtained using the quantikine.
Product name mouse prolactin elisa kit catalog number elm prolactin kit components component size description prolactin microplate item a 96 wells 12 strips x 8 wells coated with antimouse prolactin. Mouse prolactin elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 0. The diasource prolactin elisa kit is a solid phase enzymelinked immunosorbent assay elisa based on the sandwich principle. Kit components and assay qc data details of human prolactin elisa kit picokine the quality control qc data in this section is obtained from. This assay offers specific and sensitive analysis of prolactin in human serum and plasma, and has not been validated for other types of samples. The use of biological matrixes other than blood is receiving increasing interest in the study of hormones, due to the lower invasiveness in collection. These results indicate that this kit can be used to determine relative mass values for natural human. Rat prolactin elisa kit molecular innovations, inc. Human prolactin is a single chain polypeptide hormone with a molecular weight of approximately 23,000 daltons. Prolactin may have a role in breast cancer development, with higher prolactin levels correlating with postmenopausal breast cancer risk. Elisa intra assay consistency is measured using wells from the same plateassay kit. The assay will exclusively recognize both natural and recombinant hu prolactin.